ToolerWork
Biology

RNA Sequence Analyzer

Measure RNA length, A/U/G/C composition, GC content and ambiguous nucleotide counts.

Your sequence

Paste raw letters or one FASTA record

Up to 100,000 bases or residues. Whitespace and a single FASTA header are accepted. Invalid characters are flagged.

Sequences are processed locally and are not uploaded or saved by this tool.

Your result, clearly explained

Paste a sequence or load the example, then run the tool. Your sequence stays in this browser.

How to use

  1. Paste RNA

    Enter A, C, G and U, with supported IUPAC ambiguity letters.

  2. Analyze composition

    Count bases and calculate the known-base GC and AU percentages.

  3. Export statistics

    Copy or download the RNA report for your workflow.

Why ToolerWork?

Private by design

Sequence calculations run inside your browser.

Clear assumptions

Strand direction, code table and approximation limits are explained.

Related Tools

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DNA to RNA Transcription Tool

Convert coding or template DNA into an RNA sequence with explicit strand direction.

GC Content Calculator

Calculate DNA GC percentage, AT percentage and base counts with clear handling of ambiguous bases.

Codon Lookup Tool

Look up a DNA or RNA codon to find its amino acid, abbreviation and stop-codon status.

Why use this tool?

  • Validate RNA input without silently replacing DNA letters.
  • See uracil and ambiguous-base counts separately.
  • Compare nucleotide composition before downstream analysis.

Method & assumptions

The analyzer counts RNA letters in the submitted strand. GC% = 100 × (G + C) / (A + C + G + U); AU% uses the same known-base denominator. Ambiguous positions remain in the total length but are excluded from these percentages.

References: NCBI genetic codes

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Frequently asked questions

Why is T rejected?

This analyzer expects RNA, which uses U. Convert DNA through the DNA to RNA tool, choosing the correct strand, before analyzing its transcript.

Can I enter a FASTA sequence?

Yes. One header line starting with > is accepted at the beginning. The remaining lines are treated as the sequence.

Are ambiguous RNA bases included in length?

Yes. They count as positions in the total length and appear in the ambiguous count, but are excluded from GC and AU denominators.

Does this predict RNA secondary structure?

No. This tool reports sequence composition only. It does not calculate folding, free energy or stem-loop structures.

Does a high GC percentage prove an RNA is stable?

No. Stability depends on structure, sequence context and experimental conditions as well as composition.