ToolerWork
Biology

DNA to RNA Transcription Tool

Convert coding or template DNA into an RNA sequence with explicit strand direction.

Your sequence

Paste raw letters or one FASTA record

Up to 100,000 bases or residues. Whitespace and a single FASTA header are accepted. Invalid characters are flagged.

Sequences are processed locally and are not uploaded or saved by this tool.

Your result, clearly explained

Paste a sequence or load the example, then run the tool. Your sequence stays in this browser.

How to use

  1. Enter DNA

    Paste raw DNA or a single FASTA record.

  2. Identify the strand

    Choose coding 5′→3′, template 3′→5′, or template 5′→3′.

  3. Generate RNA

    Read the RNA output in the 5′→3′ direction and export it.

Why ToolerWork?

Private by design

Sequence calculations run inside your browser.

Clear assumptions

Strand direction, code table and approximation limits are explained.

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Reverse Complement Generator

Generate the reverse complement of DNA, including IUPAC ambiguity codes.

Why use this tool?

  • Avoid confusing coding and template DNA.
  • Keep strand orientation explicit in your transcription workflow.
  • Preserve IUPAC ambiguity codes instead of inventing bases.

Method & assumptions

For coding DNA, T is replaced by U. A template entered 3′→5′ is complemented; a template entered 5′→3′ is reverse-complemented. The output is always RNA in the 5′→3′ direction. This is a sequence transformation, without splicing, capping or polyadenylation.

References: NCBI genetic codes

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Frequently asked questions

Is DNA to RNA just replacing T with U?

That is correct for the coding strand. A template strand must also be complemented, and reversed if entered 5′→3′.

Which direction is the RNA output?

The output is always written 5′→3′. Choose the direction of your input template carefully.

Does this produce mature mRNA?

Only if your input already represents the appropriate processed coding sequence. The tool does not remove introns or add a cap or poly-A tail.

Are ambiguous DNA letters supported?

Yes. IUPAC ambiguity letters are preserved or complemented according to the selected strand mode. N remains N.

Why is U rejected in my DNA input?

DNA input uses T. Accepting U silently could hide a sequence-type mistake. Use the RNA Sequence Analyzer for existing RNA.